Plasmid Stability: Plasmid Loss Rate of DHB5 in Kanamycin-containing Medium

Based on the SwarmLabs virtual experiment engine, this paper conducts a cross-analysis of the plasmid dynamics scenario for E. coli DH5α in an unknown medium. The scenario employs an unknown kinetic model and leverages a GP surrogate model with an active learning strategy to extract the optimal parameter combination from the minimal number of experiments.

Experimental Scenario: Plasmid Stability Decay

This scenario simulates the growth behavior of E. coli DH5α in an unknown medium, with the core model describing unknown kinetics. Key parameters include the specific growth rate μ_max, half-saturation constant Ks, and others.

Strain Profile: Escherichia coli DH5α

Escherichia coli DH5α is a model strain commonly used in plasmid dynamics research.

Medium formulation: Unknown

Unknown is the standard growth medium for *E. coli* DH5α.

Cross-analysis: Plasmid dynamics of E. coli DH5α × Unknown

Combining the genomic features of Escherichia coli DH5α (phylum: Pseudomonadota) with unknown nutrient components enables precise prediction of growth curves in plasmid dynamics scenarios within a virtual environment. This combination covers the complete parameter space, ranging from a maximum specific growth rate of 0.81 to a half-saturation constant of 0.004.

Recommended Parameters for Virtual Experiments

Based on the scenario × strain × culture medium combination, the SwarmLabs virtual experiment engine can automatically execute the following workflow:

• Fit the unknown dynamics curve using a GP surrogate model (noise_var=0.03²)
• Through the active learning strategy, reduced from 16 runs in a full factorial experiment to ~12.
• VirtualLab Guard Tri-State Gating: Regions with prediction confidence below the threshold are automatically flagged as "verification required"
• Output the fitted curve with uncertainty intervals, with an expected R² > 0.95 (consistent with Monod 1949).

From Literature to Virtual Experiments: Plasmid Stability: Plasmid Loss Rate of DHB5 in Kanamycin-Containing Medium

Through the SwarmLabs virtual experiment engine, researchers can rapidly evaluate plasmid dynamics of *E. coli* DH5α in uncharacterized media without conducting actual wet-lab experiments. This data-driven research model compresses traditional R&D cycles that would typically take weeks into just hours, providing a novel technical pathway for microbiology research and industrial fermentation optimization.